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Ocular surface diseases, such as Stevens-Johnson syndrome, chemical and thermal burns, and inflammatory lesions damage the corneal, limbal, and conjunctival epithelia, often resulting in significant visual morbidity. Cultivated limbal stem cell transplantation has been used in the treatment of limbal stem cell deficiency1-3. The substrates used for the development of ocular surface tissue-equivalents include human amniotic membrane (HAM), collagen gel and fibrin glue, of which HAM is currently the most commonly used substrate for ocular surface transplantation1-9.
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